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Erin Anderson, MSc
Erin completed both a M.Sc. and B.Sc. at the University of Guelph. She previously worked as a laboratory technician for Dr. Joseph Lam (UofG) for nine years and Dr. Robert Mullen (UofG) for two years before deciding to complete her Ph.D., where she currently holds a NSERC CGS-D award. Erin’s project aims to understanding the mechanisms behind the high-level antimicrobial resistance of P. aeruginosa biofilms. She aims to characterize several β-lactamases that are responsible for the resistance to penicillin antibiotics and, using a mass spectrometry approach, investigate the impact these enzymes have on biofilm antimicrobial resistance. In addition, Erin is studying what impact growth as a biofilm and in particular the presence of the β-lactamases, may have on the composition of the peptidoglycan layer in the Gram-negative cell wall. |
Erin's Publications
Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.
Anderson EM, Shaji Saji N, Anderson AC, Brewer D, Clarke AJ, Khursigara CM. mSystems. 2022 Jun 28;7(3):e0015622. doi: 10.1128/msystems.00156-22. Epub 2022 May 12. PMID: 35545925; PMCID: PMC9239049.
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics.
Anderson EM, Greenwood NA, Brewer D, Khursigara CM. J Vis Exp. 2020 Oct 13;(164)
Peptidoglycomics reveals compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa.
Anderson EM, Sychantha D, Brewer D, Clarke AJ, Geddes-McAlister J, Khursigara CM.
J Biol Chem. 2020 Jan 10;295(2):504-516.
A Genotypic Analysis of Five P. aeruginosa Strains after Biofilm Infection by Phages Targeting Different Cell Surface Receptors.
Pires DP, Dötsch A, Anderson EM, Hao Y, Khursigara CM, Lam JS, Sillankorva S, Azeredo J. Front Microbiol. 2017 Jun 30;8:1229.
Regulatory Phosphorylation of Bacterial-Type PEP Carboxylase by the Ca2+-Dependent Protein Kinase RcCDPK1 in Developing Castor Oil Seeds.
Ying S, Hill AT, Pyc M, Anderson EM, Snedden WA, Mullen RT, She YM, Plaxton WC.
Plant Physiol. 2017 Jun;174(2):1012-1027.
Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase.
Mimura M, Zallot R, Niehaus TD, Hasnain G, Gidda SK, Nguyen TN, Anderson EM, Mullen RT, Brown G, Yakunin AF, de Crécy-Lagard V, Gregory JF 3rd, McCarty DR, Hanson AD. Plant Cell. 2016 Oct;28(10):2683-2696.
The Calcium-Dependent Protein Kinase RcCDPK2 Phosphorylates Sucrose Synthase at Ser11 in Developing Castor Oil Seeds.
Fedosejevs ET, Gerdis SA, Ying S, Pyc M, Anderson EM, Snedden WA, Mullen RT, She YM, Plaxton WC. Biochem J. 2016 Oct 15;473(20):3667-3682.
Biochemical and Molecular Characterization of RcSUS1, a Cytosolic Sucrose Synthase Phosphorylated in vivo at Serine 11 in Developing Castor Oil Seeds.
Fedosejevs ET, Ying S, Park J, Anderson EM, Mullen RT, She YM, Plaxton WC.
J Biol Chem. 2014 Nov 28;289(48):33412-24.
LPS Quantitation Procedures.
Lam JS, Anderson EM, Hao Y. Methods Mol Biol. 2014;1149:375-402.
Evaluation of the Ability of C. albicans to Form Biofilm in the Presence of Phage-Resistant Phenotypes of P. aeruginosa.
Pires DP, Silva S, Almeida C, Henriques M, Anderson EM, Lam JS, Sillankorva S, Azeredo J. Biofouling. 2013;29(10):1169-80.
Rhamnosyltransferase Genes migA and wapR are Regulated in a Differential Manner to Modulate the Quantities of Core oligosaccharide Glycoforms Produced by Pseudomonas aeruginosa.
Kocíncová D, Ostler SL, Anderson EM, Lam JS. J Bacteriol. 2012 Aug;194(16):4295-300.
A Cationic Lumen in the Wzx Flippase Mediates Anionic O-antigen Subunit Translocation in Pseudomonas aeruginosa PAO1.
Islam ST, Fieldhouse RJ, Anderson EM, Taylor VL, Keates RA, Ford RC, Lam JS. Mol Microbiol. 2012 Jun;84(6):1165-76.
Synthesis of Multiple Pseudomonas aeruginosa Biofilm Matrix Exopolysaccharides is Post-Transcriptionally Regulated.
Ma L, Wang J, Wang S, Anderson EM, Lam JS, Parsek MR, Wozniak DJ. Environ Microbiol. 2012 Aug;14(8):1995-2005.
Dual Conserved Periplasmic Loops Possess Essential Charge Characteristics that Support a Catch-and-Release Mechanism of O-antigen Polymerization by Wzy in Pseudomonas aeruginosa PAO1.
Islam ST, Gold AC, Taylor VL, Anderson EM, Ford RC, Lam JS. J Biol Chem. 2011 Jun 10;286(23):20600-5.
Ssg, a Putative Glycosyltransferase, Functions in Lipo- and Exopolysaccharide Biosynthesis and Cell Surface-Related Properties in Pseudomonas alkylphenolia.
Veeranagouda Y, Lee K, Cho AR, Cho K, Anderson EM, Lam JS. FEMS Microbiol Lett. 2011 Feb;315(1):38-45.
Genetic and Biochemical Analyses of the Pseudomonas aeruginosa Psl Exopolysaccharide Reveal Overlapping Roles for Polysaccharide Synthesis Enzymes in Psl and LPS Production.
Byrd MS, Sadovskaya I, Vinogradov E, Lu H, Sprinkle AB, Richardson SH, Ma L, Ralston B, Parsek MR, Anderson EM, Lam JS, Wozniak DJ. Mol Microbiol. 2009 Aug;73(4):622-38.
The Structural Basis for Catalytic Function of GMD and RMD, Two Closely Related Enzymes from the GDP-D-Rhamnose Biosynthesis Pathway.
King JD, Poon KKH, Webb NA, Anderson EM, McNally DJ, Brisson JR, Messner P, Garavito RM, LamJS. FEBS J. 2009 May;276(10):2686-2700.
Structure of the Lipopolysaccharide Core of Vibrio vulnificus Type Strain 27562.
Vinogradov E, Wilde C, Anderson EM, Nakhamchik A, Lam JS, Rowe-Magnus DA. Carbohydr Res. 2009 Mar 10;344(4):484-90.
Flagellin Glycosylation in Pseudomonas aeruginosa PAK Requires the O-antigen Biosynthesis Enzyme WbpO.
Miller WL, Matewish MJ, McNally DJ, Ishiyama N, Anderson EM, Brewer D, Brisson JR, Berghuis AM, Lam JS. J Biol Chem. 2008 Feb 8;283(6):3507-18.
Structural Studies of FlaA1 from Helicobacter pylori Reveal the Mechanism for Inverting 4,6-Dehydratase Activity.
Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM. J Biol Chem. 2006 Aug 25;281(34):24489-95.
ARC1 is an E3 Ubiquitin Ligase and Promotes the Ubiquitination of Proteins During the Rejection of Self-incompatible Brassica Pollen.
Stone SL, Anderson EM, Mullen RT, Goring DR. Plant Cell. 2003 Apr;15(4):885-98.
Pseudomonas aeruginosa Alters Peptidoglycan Composition under Nutrient Conditions Resembling Cystic Fibrosis Lung Infections.
Anderson EM, Shaji Saji N, Anderson AC, Brewer D, Clarke AJ, Khursigara CM. mSystems. 2022 Jun 28;7(3):e0015622. doi: 10.1128/msystems.00156-22. Epub 2022 May 12. PMID: 35545925; PMCID: PMC9239049.
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics.
Anderson EM, Greenwood NA, Brewer D, Khursigara CM. J Vis Exp. 2020 Oct 13;(164)
Peptidoglycomics reveals compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa.
Anderson EM, Sychantha D, Brewer D, Clarke AJ, Geddes-McAlister J, Khursigara CM.
J Biol Chem. 2020 Jan 10;295(2):504-516.
A Genotypic Analysis of Five P. aeruginosa Strains after Biofilm Infection by Phages Targeting Different Cell Surface Receptors.
Pires DP, Dötsch A, Anderson EM, Hao Y, Khursigara CM, Lam JS, Sillankorva S, Azeredo J. Front Microbiol. 2017 Jun 30;8:1229.
Regulatory Phosphorylation of Bacterial-Type PEP Carboxylase by the Ca2+-Dependent Protein Kinase RcCDPK1 in Developing Castor Oil Seeds.
Ying S, Hill AT, Pyc M, Anderson EM, Snedden WA, Mullen RT, She YM, Plaxton WC.
Plant Physiol. 2017 Jun;174(2):1012-1027.
Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase.
Mimura M, Zallot R, Niehaus TD, Hasnain G, Gidda SK, Nguyen TN, Anderson EM, Mullen RT, Brown G, Yakunin AF, de Crécy-Lagard V, Gregory JF 3rd, McCarty DR, Hanson AD. Plant Cell. 2016 Oct;28(10):2683-2696.
The Calcium-Dependent Protein Kinase RcCDPK2 Phosphorylates Sucrose Synthase at Ser11 in Developing Castor Oil Seeds.
Fedosejevs ET, Gerdis SA, Ying S, Pyc M, Anderson EM, Snedden WA, Mullen RT, She YM, Plaxton WC. Biochem J. 2016 Oct 15;473(20):3667-3682.
Biochemical and Molecular Characterization of RcSUS1, a Cytosolic Sucrose Synthase Phosphorylated in vivo at Serine 11 in Developing Castor Oil Seeds.
Fedosejevs ET, Ying S, Park J, Anderson EM, Mullen RT, She YM, Plaxton WC.
J Biol Chem. 2014 Nov 28;289(48):33412-24.
LPS Quantitation Procedures.
Lam JS, Anderson EM, Hao Y. Methods Mol Biol. 2014;1149:375-402.
Evaluation of the Ability of C. albicans to Form Biofilm in the Presence of Phage-Resistant Phenotypes of P. aeruginosa.
Pires DP, Silva S, Almeida C, Henriques M, Anderson EM, Lam JS, Sillankorva S, Azeredo J. Biofouling. 2013;29(10):1169-80.
Rhamnosyltransferase Genes migA and wapR are Regulated in a Differential Manner to Modulate the Quantities of Core oligosaccharide Glycoforms Produced by Pseudomonas aeruginosa.
Kocíncová D, Ostler SL, Anderson EM, Lam JS. J Bacteriol. 2012 Aug;194(16):4295-300.
A Cationic Lumen in the Wzx Flippase Mediates Anionic O-antigen Subunit Translocation in Pseudomonas aeruginosa PAO1.
Islam ST, Fieldhouse RJ, Anderson EM, Taylor VL, Keates RA, Ford RC, Lam JS. Mol Microbiol. 2012 Jun;84(6):1165-76.
Synthesis of Multiple Pseudomonas aeruginosa Biofilm Matrix Exopolysaccharides is Post-Transcriptionally Regulated.
Ma L, Wang J, Wang S, Anderson EM, Lam JS, Parsek MR, Wozniak DJ. Environ Microbiol. 2012 Aug;14(8):1995-2005.
Dual Conserved Periplasmic Loops Possess Essential Charge Characteristics that Support a Catch-and-Release Mechanism of O-antigen Polymerization by Wzy in Pseudomonas aeruginosa PAO1.
Islam ST, Gold AC, Taylor VL, Anderson EM, Ford RC, Lam JS. J Biol Chem. 2011 Jun 10;286(23):20600-5.
Ssg, a Putative Glycosyltransferase, Functions in Lipo- and Exopolysaccharide Biosynthesis and Cell Surface-Related Properties in Pseudomonas alkylphenolia.
Veeranagouda Y, Lee K, Cho AR, Cho K, Anderson EM, Lam JS. FEMS Microbiol Lett. 2011 Feb;315(1):38-45.
Genetic and Biochemical Analyses of the Pseudomonas aeruginosa Psl Exopolysaccharide Reveal Overlapping Roles for Polysaccharide Synthesis Enzymes in Psl and LPS Production.
Byrd MS, Sadovskaya I, Vinogradov E, Lu H, Sprinkle AB, Richardson SH, Ma L, Ralston B, Parsek MR, Anderson EM, Lam JS, Wozniak DJ. Mol Microbiol. 2009 Aug;73(4):622-38.
The Structural Basis for Catalytic Function of GMD and RMD, Two Closely Related Enzymes from the GDP-D-Rhamnose Biosynthesis Pathway.
King JD, Poon KKH, Webb NA, Anderson EM, McNally DJ, Brisson JR, Messner P, Garavito RM, LamJS. FEBS J. 2009 May;276(10):2686-2700.
Structure of the Lipopolysaccharide Core of Vibrio vulnificus Type Strain 27562.
Vinogradov E, Wilde C, Anderson EM, Nakhamchik A, Lam JS, Rowe-Magnus DA. Carbohydr Res. 2009 Mar 10;344(4):484-90.
Flagellin Glycosylation in Pseudomonas aeruginosa PAK Requires the O-antigen Biosynthesis Enzyme WbpO.
Miller WL, Matewish MJ, McNally DJ, Ishiyama N, Anderson EM, Brewer D, Brisson JR, Berghuis AM, Lam JS. J Biol Chem. 2008 Feb 8;283(6):3507-18.
Structural Studies of FlaA1 from Helicobacter pylori Reveal the Mechanism for Inverting 4,6-Dehydratase Activity.
Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM. J Biol Chem. 2006 Aug 25;281(34):24489-95.
ARC1 is an E3 Ubiquitin Ligase and Promotes the Ubiquitination of Proteins During the Rejection of Self-incompatible Brassica Pollen.
Stone SL, Anderson EM, Mullen RT, Goring DR. Plant Cell. 2003 Apr;15(4):885-98.